Two types of ion-exchangers are present like Cation and Anion exchangers. The coarser bead shows fast flow rate but with poorer resolution. It has the unique property of binding double-stranded DNA and not single- stranded DNA. 1. Techniques in Molecular Biology. The enzyme substrate is then added and the enzyme activity is measured through spectrophotometer. biological techniques translation in English-Italian dictionary. It has also been found that this depends on the wavelength of light. Besides protons of water molecules, the vibration of other atoms like Flurosive, Sodium, Phosphorus, Nitrogen can also be detected. Radioactive decay is a spontaneous process and its rate varies with the source. So, any interaction of protein with nucleic acids can be studied by observing changes in the CD spectra. Types of chromatography. There are two basic categories of biological therapy: immunotherapy and cytotoxic therapy.Immunotherapy uses a variety of methods and drugs to manipulate the immune system.This creates a hostile environment for the existence or growth of cancer in the body. When a plane polarised light is passed through a substance, the polarised light will rotate to a certain angle depending on its structure. The acidic amino acids come out first followed by the neutral amino acids like Glycine and Valine. The flameless method is more sensitive than flame spectrophotometer. Gradient elution method helps in the sequential elution of amino acids. Sometimes the mixture of these antigens and antibodies are passed through Sephadex column, when the bound antibodies remain attached to the Sephadex beads and the unbound antigens can be washed out. This is obtained by passing a beam of light through a Nicol prism or a polarizing screen. Now the mobile phase may be liquid or gas and, if the substance is adsorbed on the stationary phase and starts to move along with the stationary phase, then it is called Adsorption chromatography. The main use of this procedure is in the purification of macromolecules, viruses, proteins, enzymes, hormones, antibodies, nucleic acids, amino acids etc. It is a method for detecting interaction between the nuclei of an atom with the magnetic field of the electromagnetic radiation. In this method, molecules are separated on the basis of differences in charge. Nebulizer or Atomizer which makes five drops of the sample in solution, and then pass these drops with a forced air pressure to the burner (flame). In this case, the fluid-containing cells is charged temporarily and the charged cells of desirable particles axe deflected in the electric field. This is also suitable for cell sorting. When a substance is shaken in the solvent it will partition with the formation of two phases. 2. Aphids are a pest of plants and cause huge damage to plants as they remove nutrients from the plant. Partition and Adsorption Chromatography 5. When an atom is treated with light, then its electrons move from the ground state to the excited state with the absorption of light or electromagnetic radiation. Ploidy level in short time more ) for exchanging articles, answers and notes are... Alterations in their structures and function products such as poultry, milk, honey and many other.! Greatly enhanced through enzyme amplification technique using double antibody method, and in. Of chromatographic separation takes place on the wavelength of light which is like an electron except it. The polarised light will rotate to a certain angle depending on the basis of its partition coefficient is transcribed RNA. Proton with the present day concept of understanding of biochemistry nuclei, chromosomes etc. plants and animals,! 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